UJI KANDUNGAN FENOLIK TOTAL DAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH ASAM PAYA (Eleiodoxa conferta (Griff.) Burret) TERHADAP Staphylococcus aureus.
Abstract
Asam paya (Eleiodoxa conferta (Griff.) Burret) contains phenolic compounds which can function as antibacterial agents against Staphylococcus aureus. Extraction using soxhlet with multilevel polarity, namely n-hexane and ethanol. The antibacterial activity test by TLC contact-bioautography showed that the phenolic compounds in the ethanol extract had antibacterial activity, and the diffusion test results showed that the ethanol extract had a larger inhibition zone diameter than the n-hexane extract. The total phenolic compound of extract was determined using the Folin-Ciocalteu reagent. The total phenolic compounds of Eleiodoxa conferta (Griff.) Burret. ethanolic extract is 7,326± 0,0989 mg GAE/gram sample. The ethanol extract was fractionated using a separating funnel, followed by purification with preparative TLC. The phenolic isolates obtained were tested for purity by three eluent TLC. The results of antibacterial activity diffution test and TLC contact-bioautography test showed that phenolic isolates had antibacterial activity against Staphylococcus aureus.
References
Afriani S., Indiawati N., Destiarti L.,
Arianie L., 2014, Uji Aktivitas
Antioksidan Daging Buah Asam
Paya (Eleiodoxa conferta Burret)
Dengan Metode DPPH dan Tiosianat,
JKK, 3 (1), 49-56
Kristianto Y.B., Sulistyarini, I., Suharsanti,
R., 2019, Uji Aktivitas Antibakteri
Ekstrak Etanol, Air Buncis
(Phaseolus vulgaris L.) dan Fraksifraksinya
Terhadap Pertumbuhan
Bakteri Staphylococcus aureus,
Media Farmasi Indonesia, 14 (2),
-1550
Lenny, S., 2006, Senyawa Terpenoida dan
Steroida, USU Press, Medan
Mhaske, M., Samad, B. N., Jawade, R.,
Bhansali, A., 2012, Chemical
Agents in Control of Dental Plaque
in Dentistry: An Overview of
Current Knowledge and Future
Challenges, Pelagia Research
Library, 3 (1), 268- 272
Mongkolsilp,M., Pongbupakit,I., Sae, L.N.
and Sitthithaworn,W., 2004, Radical
scavenging activity and total
phenolic content of medicinal plants
used in primary health care, SWU. J
Pharm. Sci., 9, 32-35
Neal, M. J., 2006, At a Glance
Farmakologis Medis, Edisi kelima,
Diterjemahkan oleh Safitri, A.,
Penerbit Erlangga, Jakarta
Oliver, S. P., B. E. Gillespie, M. J. Lewis,
S. J. Ivey, R. A. Almeida, D. A.
Luther, D. L. Johnson, K. C. L.amar,
H. D. Moorehead and H. H.Dowlen.,
, Efficacy Of A New Premilking
Teat Disinfectant Containing A
Phenilic Combination For The
Prevention of Mastitis, J. Dairy Sci,
(3), 1545-1549
Retnowati, Y., Nurhayati, B., Nona, W.P.,
, Pertumbuhan Bakteri
Staphylococcus aureus pada Media
yang Diekspos dengan Infus Daun
Sambiloto (Andrographis
paniculata), Saintek, 6 (2)
Safitri G.L., Wibowo M.A., Indiawati N.,
, Uji Aktivitas Ekstrak Kasar
Buah Asam Paya (Eleiodoxa
conferta (Griff.) Burret) Terhadap
Bakteri Staphylococcus aureus Dan
Salmonella thypi, JKK, 6 (1), 17-20
Sari, R. P., Nazrun, Surtina, Mahardika, R.
G., 2019, Uji Fitokimia dan Aktivitas
Antibakteri pada Air Kelubi
(Eleiodoxa conferta) terhadap bakteri
Staphylococcus aureus, Prosiding
Seminar Nasional Penelitian &
Pengabdian Pada Masyarakat, ISBN:
-602-61545-0-7
Sasmito, S.P.T., Wulandari, Wulansari,
E.D., 2020, Senyawa Fenolik Dalam
Fraksi Aktif Kulit Buah Eleiodoxo
conferta Yang Berpotensi
Antibakteri Terhadap Bakteri
Streptococcus mutans, Pharmacy
Medical Journal, 3(1), 28-33
Sudigdodadi, S., 2014, Mikrobiologi pada
Infeksi Kulit, Universitas Padjajaran,
Bandung
Vermerris, W., Nicholson, R., 2006,
Phenolic Compound Biochemistry,
The Springer, Netherlands
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