Formulation and Antioxidant Activity Test of Michelia champaca Linn. Flower Methanol Extract Gel Using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method

Keywords: antioxidant activity, Michelia champaca flower, methanol extract, gel., antioxidant activity, Michelia champaca flower, methanol extract, gel

Abstract

Michelia champaca, a tropical evergreen flower utilized for religious rituals in Bali, exhibits notable level of flavonoid with potent antioxidant properties. This study aims to investigate the antioxidant activity of gel formulation using methanol extract of Michelia champaca flowers. The gel formulations were prepared using varying concentration of the extract (5%, 7,5%, and 10%), and underwent physical evaluations. Statistical analysis was performed using SPSS version 22 at a 95% confidence level. The results showed gel formulations exhibit desirable physical characteristics, indicated by the presence of homogenous yellow-brown gel with the fragrance of extract, a spreadability range of 4.83-6.90 cm, adhesion time of 2.293-2.616 seconds, and pH of 5. The dispersion test revealed no significant difference between all formulations. The antioxidant activity was determined using DPPH radical scavenging assay, with IC50 values indicating the efficacy of gel. The IC50 values for FI, FII, and FIII were 49,859 µg/ml, 23.774 µg/ml, and 6.626 µg/ml, respectively, compared to the IC50 value of vitamin C 4,861 µg/ml. These results indicate that the Michelia champaca methanol extract gel possesses potent antioxidant activity. Moreover, the higher concentration of the extract exhibited a smaller IC50 value, indicating increased efficacy in scavenging free radicals.

Author Biographies

Ni Nyoman Yudianti Mendra, Universitas Mahasaraswati Denpasar

Departemen Farmasetika, Fakultas Farmasi, Universitas Mahasaraswati Denpasar

Ni Made Puspayanti, Prodi Diploma Tiga Farmasi, Fakultas Farmasi, Universitas Mahasaraswati Denpasar

Prodi Diploma Tiga Farmasi

Ni Putu Udayana Antari, Universitas Mahasaraswati Denpasar

Departemen Farmasi Sosial, Fakultas Farmasi, Universitas Mahasaraswati Denpasar

I Gede Made Suradnyana, Universitas Mahasaraswati Denpasar

Departemen Farmasetika, Fakultas Farmasi, Universitas Mahasaraswati Denpasar

I Wayan Surya Rahadi , Universitas Mahasaraswati Denpasar

Departemen Kimia Farmasi, Fakultas Farmasi, Universitas Mahasaraswati Denpasar

Published
2023-09-12