Benefits of Stevia (Stevia rebaudiana B.) and Sirih (Piper betle L.) Leaves Combination Againts Dental Care
Abstract
Oral health is the most important part, to improve its hygiene by maintaining teeth. Cavities are a common problem. Betel leaf as an antibacterial. Stevia leaves as bacteriostatic and bactericidal also eliminate the cause of tooth decay and gingivitis. The purpose of the study was to determine the antibacterial activity of toothpaste combined with betel leaf extract with stevia leaves against the growth of Streptococcus mutans bacteria. The study used an experimental method consisting of making betel leaf and stevia leaf extracts using the maceration method. Preparation of toothpaste with a combination of betel leaf extract with stevia leaves at a concentration of 15% with the ratio of betel leaves and stevia leaves, namely formula 1 (5%: 10%), formula 2 (7.5%: 7.5%), formula 3 (10%: 5%). Testing antibacterial activity by measuring the clear zone using the disc paper method with blood agar media. Data analysis using ANOVA and Paired T test. The results of the study obtained formula (5%: 10%) clear zone of 16.42 mm, formula (7.5%: 7.5%) of 17.24mm, formula (10%: 5%) of 17.94mm, positive control of 18.66mm, negative control of 2.75mm. Conclusion 10:5 combination toothpaste has antibacterial activity.
References
Anas, R., Kurniawan, K., & Puspitasari, Y.
(2018). Perbedaan Daya Hambat
Antibakteri Antara Ekstrak Daun
Sirih Merah (Piper crocatum) dan
Ekstral Daun Sirih Hijau (Piper betle
L.) terhadap Bakteri Streptococcus
mutans. Jurnal Ilmiah As-Syifaa,
(1).
https://doi.org/10.33096/jifa.v10i1.3
Asrina, R. (2019). Formulasi Stabil Pasta
Gigi Dari Ekstrak Etanol Daun
Gamal (Gliricida sepium) sebagai
Pencegah Karies Gigi. Jurnal
Farmasi Sandi Karsa, 5(2), 99–104.
https://doi.org/10.36060/jfs.v5i2.50
Aziz, Y. S. (2019). Standarisasi Parameter
Non Spesifik Simplisia Rimpang
Kunyit (Curcumae Domestica
Rizhoma) dan Temulawak (Curcuma
xanthorrhiza Roxb.) di Kabupaten
Ponorogo. Jurnal Delima Harapan,
(2), 89–94.
https://doi.org/10.31935/delima.v6i2
.84
Davis, W. W., & Stout, T. R. (1971). Disc
plate method for microbiological
antibiotic tests. II. A new procedure
offering improved accuracy. Applied
Microbiology, 22(4), 666-670.
Ilyas, I. L., Aliah, A. I., Ulandari, S. A.,
Turi, D., & Daun, D. (2023). Uji
Aktivitas Obat Kumur Ekstrak Daun
Turi (Sesbania grandiflora L .) dan
Daun (Piper betle L.) Terhadap
Bakteri Staphylococcus aureus. 4, 8–
Hilma S, Study of Green Betel Leaf (Piper
betle L) as Antibacterial, Journal of
Veterinary Science, 2022
Magdalena, R., & Angela Krisanti, M.
(2019). Analisis Penyebab dan Solusi
Rekonsiliasi Finished Goods
Menggunakan Hipotesis Statistik
dengan Metode Pengujian
Independent Sample T-Test di
PT.Merck, Tbk. Jurnal Tekno, 16(2),
–48.
https://doi.org/10.33557/jtekno.v16i
623
Marlina, D., & Rosalini, N. (2017).
Formulasi Pasta Gigi Gel Ekstrak
Daun Sukun (Artocarpus altilis)
dengan Natrium CMC sebagai
Gelling Agent dan Uji Kestabilan
Fisiknya. Jurnal Kesehatan
Palembang (JJP), 12(1), 36–50.
Nurhamidah, N., Sofia Ramadhan, E.,
Asmawati, A., & Juni, J. (2016).
Hubungan Status Kesehatan Gigi dan
Mulut dengan Prestasi Belajar
Siswa/I Sd Negeri 2 Sangga Beru
Kecamatan Gunung Meriah
Kabupaten Aceh Singkil. Jurnal
Kesehatan Gigi, 3(2),35–40.
https://doi.org/10.31983/jkg.v3i2.17
Oktami, E., Lestari, F., & Aprilia, H.
(2021). Studi Literatur Uji
Stabilitas Sediaan Farmasi Bahan
Alam. Prosiding Farmasi
Universitas Islam Bandung, 7(1), 72–
http://dx.doi.org/10.29313/.v7i1.261
Putri, A. V. A. A., Hafida, N., & Megawati,
V. (2017). Pengaruh daya antibakteri
ekstrak daun stevia (Stevia
rebaudiana bertoni) pada konsentrasi
%, 10%, 20%, 40%, dan 80%
terhadap Streptococcus mutans (in
vitro). Jurnal Ilmu Kedokteran Gigi,
(1), 9–14.
Putri Efendi, A. P., Sholikah, N., &
Ismawati, R. (2020). Pembuatan
Hand Sanitizer Alami dengan
Memanfaatkan Tumbuhan Daun
Sirih Di Rw 04 Desa Setia Mekar.
Abdiraja (Jurnal Pengabdian
Kepada Masyarakat), 1(1), 29.
https://doi.org/10.31002/abdipraja.v1
i1.3197hp/jkma/article/view/114/120
Ramayanti, S., & Purnakarya, I. (2013).
Peran Makanan terhadap Kejadian
Karies Gigi. Jurnal Kesehatan
Masyarakat, 7(2), 89–93.
http://jurnal.fkm.unand.ac.id/index.p
Simon, L. (2010). The Role of
Streptococcus mutans And Oral
Ecology in The Formation of ... The
Role of Streptococcus mutans And
Oral Ecology in The Formation of
Dental Caries Table of Contents The
Role of Streptococcus mutans And
Oral Ecology in The Formation of ...
Micro. 2, 1–6.
Suryani, Putri, A. E. P., & Agustyiani, P.
(2017). Formulasi dan Uji Stabilitas
Sediaan Gel Ekstrak Terpurifikasi
Daun Paliasa (Kleinhovia Hospita
L.). Pharmacon, 6(3), 157–169.
Wahidin, Farid, A. M., & Firmansyah.
(2021). Formulation and Stability
Test of Racial Chicken Egg Shell
Toothpaste (Galur sp) with Various
Concentrations of Na.cmc Fito
Medicine: Journal of Pharmacy and
Sciences, 12(2), 121-130.
Wenda, Y., Wowor, P. M., & Leman, M.A.
(2017). Uji daya hambat ekstrak daun
stevia (Stevia rebaudiana Bertoni M.)
terhadap pertumbuhan
Staphylococcus aureus secara in
vitro. E-GIGI, 5(1).
https://doi.org/10.35790/eg.5.1.2017.
Widarsih, E., & Mahdalin, A. (2017).
Formulasi Pasta Gigi Daun Sirih
(Piper betle L.) dengan Pemanis
Alami Ekstrak Daun Stevia (Stevia
rebaudiana). Urecol, 1(10), 157–162.
http://journal.ummgl.ac.id/index.php
/urecol/article/view/1322
Widayanti, N. (2014). Faktor yang
berhubungan dengan karies gigi anak
pada usia 4-6 tahun. Jurnal Berkala
Epidemiologi, 2(2), 196–205.
Willia novita, Antibacterial Activity Test of
Betel Leaf Fraction (Piper betle
L) Against the Growth of
Streptococcus mutans Bacteria In
vitro, JMJ, 2017.