PENGARUH METODE EKSTRAKSI TERHADAP KADAR FENOLIK DAN FLAVONOID TOTAL EKSTRAK TONGKOL JAGUNG (Zea mays L.)
Abstract
Abstract
Corncobs is an agricultural waste having antioxidant activity because it contains phenolic and flavonoid compounds. One of the factors improving the quality of extract is slection of method extraction. Research aims to understand the influence of a method of extraction to the total phenolic and the total flavonoid contents in corncobs extract. The corncobs are macerated for three days and in reflux at the temperature 40°C for four hours. A solvent it used is ethanol 70 %. An extract obtained is determined for the total phenolic with the method Folin-ciocalteau using gallic acid as standart while levels flavonoid determined by the addition of reagent AlCl3 using Quersetin as a standart. Total phenolic content and total flavonoid content measured use of the spectrophotometer UV-Vis. The research results showed that of suggest reflux produce the total phenolic and the total flavonoid larger compared method maceration.The stastitical results using Independent t-Test showed that different method had significant to the total phenolic corncobs extract but not significantly to the total flavonoid contents in corncobs extract.
References
BPS, 2015. Data Sensus. Jakarta: BPS. Diambil dari http//www.bps.go.id/Brs/view/id/1157 [Diakses 10 Mei 2016]
Chang CC., Yang M.H., Wen H.M., Chern J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal.
Dai, J. dan Mumper, R.J., 2010. Plant Phenolics : Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules,
Depkes RI., 1986. Sediaan Galenik. Departemen Kesehatan Republik Indonesia, Jakarta.
Depkes RI., 2008. Farmakope Herbal Indonesia Edisi I. Departemen Kesehatan Republik Indonesia, Jakarta.
Desmianty, Y., J. Ratnawati., P. Andini, 2009. Penentuan Jumlah Flavonoid Total Ekstrak Etanol Daun Buah Merah (Pandanus conoideus Lamk.). Jurusan Farmasi Universitas Jendral Achmad Yani, Jawa Barat.
Harbone, JB., 1996. Metode Fitokimia (diterjemahkan oleh Dr. K. Padmawinata), Penerbit ITB, Bandung.
Nadhiya, K., and K. Vijayalakshmi., 2014. Evaluatiom of total phenol, falvonoid contents and invitro antioxidant activity of benincasa hispida fruit extracts. International jurnal of pharmaucetical, chemical and biological sciences. Tamil Nadu, India
Ramayani, S.L., 2015. Pengaruh pH pada Kadar Total Senyawa Fenolik Ekstrak Apium graveolens L., Orthosiphon stamineus (BI.), Miq, Morinda citrifolia L. dan Pengembangannya dalam Bentuk Tablet. Tesis. Falkutas FarmasiUniversitas Gadjah Mada. Yogyakarta.
Saryana, R.V., Suryanto, E. Dan Sangi, M. S., 2014. Perbandingan Aktivitas Antioksidan Dari Tongkol Jagung (Zea mays L.) Segar dan Kering
Dengan Metode Refluks. Jurnal MIPA UNSRAT Online 3 (2): 92 96. Setyowati, W.A.E., S.R.D, Ariani., Ashadi., Bakti., Mulyani., C.P, Rahmawati., 2015. Skrining
Fitokimia Dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk.FMIPA PKIP UNS Surakarta.
Wungkana, I., Suryanto, E., dan Momuat, L., 2013. Aktivitas Antioksidan dan Tabir Surya Fraksi Fenolik dari Limba Tongkol Jagung (Zea mays L.). Jurnal Ilmiah Farmasi UNSRAT Vol. 2 No. 4 : Manado.
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