Hypnotic-Sedative Effectiveness Of Ethanol Extract Keji Beling (Strobilanthes Crispus) In Mice With Rotarod Test As An Indicator Of Motor Impairment
Abstract
Insomnia is a sleep disorder that adversely affects the quality of life, mental and physical health. Treatment of insomnia often utilizes pharmacological therapies that have side effects. Keji beling (Strobilanthes crispus) contains secondary metabolites of flavonoids, tannins, alkaloids, saponins, and triterpenoids that have potential as natural sedatives and hypnotics. This study uses the rotarod test to detect motor impairment and evaluate the effectiveness of the ethanol extract of keji beling leaves (EEKB) as a hypnotic-sedative agent in mice. Extraction of keji beling by maceration using 96% ethanol, the extract was tested on mice in four groups: diazepam 5 mg, CMC Na 1%, and EEKB 600 and 1200 mg/kgBB. The parameter measured was the number of falls on the rotarod for 30 minutes after administration. EEKB showed decreased motor balance. The 1200 mg/kgBB dose produced the highest mean number of falls (25 times), followed by the 600 mg/kgBB dose (11.25 times), similar to the diazepam 5mg (16.75 times). Statistical analysis indicated that EEKB exerted a significant hypnotic-sedative effect by modulating GABA receptors. EEKB in doses of 600 and 1200 mg/kgBB has an effectiveness comparable to diazepam as a hypnotic tranquilizer, so it has the potential to be developed as a natural-based insomnia therapy agent.
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