Effect of Xanthan Gum Concentration Variations on the Physical Properties of Breadfruit Leaf (Artocarpus altilis) Extract Serum
Abstract
Breadfruit leaves (Artocarpus altilis) contain flavonoid compounds with high antioxidant activity capable of neutralizing free radicals and preventing premature aging. In this study, breadfruit leaf extract was formulated into a serum using xanthan gum as a gelling agent. Serum was chosen for its ability to contain high concentrations of active ingredients and its rapid absorption into the skin. Xanthan gum exhibits excellent stability across a wide range of pH and temperature, and it is easily combined with other pharmaceutical ingredients. The purpose of this study was to determine the effect of various xanthan gum concentrations on the physical quality of breadfruit leaf extract serum. This experimental study compared three formulas with varying xanthan gum concentrations: Formula I (0.5%), Formula II (1%), and Formula III (2%), while the concentration of breadfruit leaf extract remained constant at 0.75% for each formula. Physical quality evaluations included organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion tests. Based on the organoleptic test, all three serum formulas showed similar results: a dark green color with a strawberry aroma and a consistency ranging from liquid to thick. For the pH test, all three formulas met the required standards; however, Formula I showed less than optimal results in terms of homogeneity. In the viscosity and spreadability tests, only Formula II met the requirements, while Formula III only fulfilled the spreadability test standards. Statistical analysis indicated that variations in xanthan gum concentration significantly affected all physical quality parameters of the breadfruit leaf extract serum. An increase in xanthan gum concentration led to higher pH, viscosity, and adhesion values, but resulted in a decrease in the spreadability of the breadfruit leaf extract serum.
References
Al-barghouthy, E. Y., Hamed, S., Mehyar, G. F., & AlKhatib, H. S. (2025). Comparative Evaluation of Spreadability Measurement Methods for Topical Semisolid Formulations/A Scoping Review. Gels, 11, 1–22. https://doi.org/10.3390/gels11121006
Anggraeni, N., Tiadeka, P., & Ratnasari, D. (2023). Formulasi Sediaan Suspensi Antasida dengan Variasi Konsentrasi Suspending Agent Xanthan Gum. Jurnal Sintesis, 4(2), 104–119. https://doi.org/10.56399/jst.v4i2.136
Arifin, A., Pakki, E., & Fitrah. (2023). Formulasi dan Uji Stabilitas Fisik Sediaan Losio Bubur Rumput Laut (Eucheuma alvarezii (Doty)) Asal Kabupaten Luwu Sulawesi Selatan. Jurnal Farmamedika, 8(2): 174–184.
Astuti, A., & Fitri, N. (2020). Formulasi Serum Anti-Aging Minyak Atsiri Lada Hitam (Piper nigrum L.) dan Uji Aktivitas Antioksidan Menggunakan Metode DPPH. Asian Journal of Innovation and Entrepreneurship, 5(1), 1–11.
Auranajwa, P., Puradewa, L., & Indriasari, C. (2025). Pengaruh Variasi Konsentrasi Xanthan Gum terhadap Mutu Fisik Serum Ekstrak Daun Matoa (Pometia pinnata). Biospektrum Jurnal Biologi, 6(1), 25–33. https://doi.org/10.33508/bios.v6i1.7420
Budiawan, A., Alfianingrum, F., Kirana, B.C., & Cahyani, E.D. (2026). Effect Of Cetyl Alcohol Variation On Formulation And Stability Of Belimbing Wuluh Leaf Extract (Averrhoa bilimbi L.) Cream. Media Farmasi Indonesia, 21(1), 59 - 71. https://doi.org/10.53359/mfi.v21i1.348
Chandra, D., & Rahmah. (2022). Uji Fisikokiimia Sediaan Emulsi, Gel, Emulgel Ekstrak Etanol Goji Berry (Lycium barbarum L.). MEDFARM: Jurnal Farmasi Dan Kesehatan, 11(2), 219–228.
Dąbrowska, A. K., Rotaru, G.-M., Derler, S., Spano, F., Camenzind, M., Annaheim, S., & Rossi, R. M. (2016). Materials Used to Simulate Physical Properties of Human Skin. Skin Research and Technology, 22, 3–14. https://doi.org/10.1111/srt.12235
Efrilia, M., Chandra, P. P. B., Whardhani, S. K., Falestin, S. L. K., Lisnawati, N., Cristian, Y. E., & Adini, S. (2025). Penetapan Kadar Flavonoid Total Ekstrak Etanol 96% Daun Sukun (Artocarpus altilis) Berdasarkan Metode Spektrofotometri UV-Vis. Jurnal Ilmiah Farmasi, 3(3), 325–338.
Ekawati, H., & Hariningsih, Y. (2023). Formulasi dan Uji Antioksidan Sediaan Serum Wajah Ekstrak Etanol. Jurnal Ilmiah Fitomedika Indonesia, 12(1), 209–216.
Ezera, J. E., Nwufo, B.T., Wapwera, J. A. (2019). Effects of Graded Quantities of Xanthan Gum on the Physicochemical and Flocculation Properties of Gum Arabic. JOJ sciences, 2(1), 555579. https://doi.org/10.19080/JOJS.2018.01.555579
Fikayuniar, L., Kusumawati, A. H., Silpia, M. P., Monafita, H., & Tusyaadah, L. (2021). Formulasi Dan Uji Efektivitas Antibakteri Sediaan Serum Antijerawat Ekstrak Etanol Daun Kemangi (Ocimum x Africanum Lour.). Jurnal Buana Farma, 1(4), 14–20.
Firmansyah, F., Khairiati, R., Muhtadi, W. K., & Chabib, L. (2022). Uji Aktivitas Antibakteri Serum Ekstrak Etanol Buah Belimbing Wuluh Terhadap Propionibacterium acnes, Staphylococcus aureus, dan Staphylococcus epidermis. Majalah Farmasi dan Farmakologi, 26(2), 69–73. https://doi.org/10.20956/mff.v26i2.18578
Gragnani, A., Cornick, S. Mac, Chominski, V., Ribeiro de Noronha, S. M., Alves Corrêa de Noronha, S. A., & Ferreira, L. M. (2014). Review of Major Theories of Skin Aging. Advances in Aging Research, 3(4), 265–284. https://doi.org/10.4236/aar.2014.34036
Hanifah, R., & Amalia, N. (2023). Uji Aktivitas Antioksidan Sediaan Serum Wajah Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) Dengan Metode DPPH. Journal of Pharmacopolium, 1(2), 27–40.
Happe, I. N., Yustiti, A. J., Ikhsan, M. K., & Yusuf, M. (2024). Formulasi Dan Uji Antioksidan Sediaan Serum Ekstrak Daun Keji Beling (Strobilanthes crispus L Blume) dengan Metode DPPH. Indonesian Health Journal, 3(2:, 118–127. https://doi.org/https://doi.org/10.56314/inhealth.v3i2.229
Hasriyani, Presticasari, H., P, N. D., & Mundriyastutik, Y. (2022). Pengaruh Variasi Konsentrasi HPMC Terhadap Kualitas Mutu Sediaan Facial Wash Gel Nanoperak Hasil Biosintesis Ekstrak Buah Pepaya (Carica papaya L.). Indonesia Jurnal Farmasi, 7(1), 63–69.
Hikmah, F. N., Malahayat, S., & Nugraha, D. F. (2023). Formulasi Dan Evaluasi Sediaan Serum Gel Ekstrak Bunga Melati (Jasminum sambac L.). Journal of Pharmaceutical Care and Sciences, 3(2), 93–108. https://doi.org/10.33859/jpcs.v3i2.248
Ministry of Health of the Republic of Indonesia (Kementerian Kesehatan RI). (2017). Farmakope Herbal Indonesia Edisi II. Jakarta: Ministry of Health of the Republic of Indonesia.
Kirana, B. C., Cahyani, E. D., & Budiawan, A. (2023). Protective Factor Evaluation of Purslane (Portulaca grandiflora) Magenta Flower Variety Herbs Extract Cream Formula. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 10(3), 379–385. https://doi.org/10.20473/jfiki.v10i32023.379-385
Kirana, V. A., Kirana, B. C., & Indriasari, C. (2025). Efektivitas Sediaan Salep Ekstrak Daun Afrika (Vernonia amydalina) terhadap Penyembuhan Luka pada Kelinci (Oryctolagus cuniculus). Biospektrum Jurnal Biologi, 6(1), 17–24. https://doi.org/10.33508/bios.v6i1.7371
Malinda, O., & Syakdani, A. (2020). Potensi Antioksidan Dalam Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Sebagai Anti-Aging. Jurnal Kinetika, 11(03), 60–65.
Matsuda, M., Hoshino, T., Yamakawa, N., Tahara, K., Adachi, H., Sobue, G., Maji, D., Ihn, H., & Mizushima, T. (2013). Suppression of UV-Induced Wrinkle Formation by Induction of HSP70 Expression in Mice. Journal of Investigative Dermatology, 133(4), 919–928. https://doi.org/10.1038/jid.2012.383
Nugraha, T. S., Sari, M., & Wasiaturrahmah, Y. (2022). Formulation and Physical Properties Test of Lotion Preparations from Breadfruit Leaf (Artocarpus altilis) Ethanol Extract. Journal of Current Pharmaceutical Sciences, 6(1), 598–603.
Nugrahaeni, F., Srifiana, Y., & Rokhman, A. N. (2021). The Effect of Increasing Xanthan Gum Concentration as a Gel Base on the Physical Properties of Sappan Wood (Caesalpinia sappan L.) Extract Hair Dye Gel. Indonesia Natural Research Pharmaceutical Journal, 6(2), 29–42.
Nurjanah, M. K., Mirawati, M., & Najib, A. (2025). Formulasi dan Evaluasi Fisik Sediaan Emulgel Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) sebagai Tabir Surya. Jurnal Mandala Pharmacon Indonesia, 11(2), 721–731. https://doi.org/10.35311/jmpi.v11i2.988
Octavia, A. D., Rizkuloh, L. R., & Adlina, S. (2025). Aktivitas Antioksidan Serum Wajah Berbasis Ekstrak Etanol Daun Sukun (Artocarpus altilis) Sebagai Produk Kosmetik Alami. Pharmacoscript, 8(2), 596–609. https://doi.org/10.36423/pharmacoscript.v8i2.2253
Priani, S. E., Budiman, S. N., Aryani, R., Fitrianingsih, S. P., & Syafnir, L. (2025). Formulasi dan Karakterisasi Sediaan Tabir Surya Mengandung Minyak Kayu Manis (Cinnamomum burmannii Nees Ex Blume). Indonesian Journal of Pharmaceutical Science and Technology, 12(1), 85–92.
Putri, Y. D., Tristiyanti, D., & Sri, Y. D. (2025). Formulation and Activity Test of Breadfruit Leaves (Artocarpus altilis (Parkinson) Fosberg) Extract Serum Spray as Antioxidant. Jurnal Sain dan Teknologi Farmasi Indonesia, 14(1), 1–8.
Rahmasari, D., Yuwanda, A., & Rahmawati, D. (2024). Formulasi dan Nilai IC50 Sediaan Krim Ekstrak Daun Sukun (Artocarpus altilis). Innovative: Journal Of Social Science Research, 4(2), 1–18.
Rahmat, S., Zuraidah, S., Deswita, D., & Leto, K. T. (2025). Skrining Fitokimia Daun Sukun (Artocarpus altilis). Jurnal Matematika dan Ilmu Pengelatuan Alam, 3(1), 1–9. https://doi.org/10.59581/konstatnta-widyakarya.v3i1.4770
Rahmavika, T., Murdiana, H. E., & Rawar, E. A. (2023). Formulasi Dan Uji Antioksidan Serum Minyak Atsiri Kulit Jeruk Nipis (Citrus aurantifolia) Menggunakan Vitamin E Metode DPPH. Pharmamedica Journal, 8(2), 209–219. https://doi.org/10.47219/ath.v8i2.294
Rowe, R. C., Sheskey, P. J., & Owen, S. C. (2009). Pharmaceutical Excipients (Fifth Edit). Pharmaceutical Press.
Sawiji, R. T., Rumbory, S., Cahyaningrum, D. A. P. W., Dewi, N. P. F. S., Nauli, M. N., Putri, A. A. I. P. A., & Suryan, N. M. L. (2024). Pengaruh Variasi Konsentrasi Gelling Agent (Xanthan Gum dan Carbopol) pada Sediaan Serum dengan bahan Aktif Retinoic Acid. Acta Holistica Pharmaciana, 6(1), 1–10. https://doi.org/10.62857/ahp.v6i1.157
Setiawan, P. A., Rahmawanty, D., & Sari, D. I. (2023). Formulasi dan Evaluasi Sifat Fisik Sediaan Serum Wajah Ekstrak Daun Singkong (Manihot esculenta) dengan Variasi Konsentrasi Xanthan Gum. Jurnal Pharmascience, 10(2), 394–404. https://doi.org/10.20527/jps.v10i2.15214
Silvia, B. M., & Dewi, M. L. (2022). Studi Literatur Pengaruh Jenis dan Konsentrasi Basis terhadap Karakteristik Masker Gel Peel Off. Jurnal Riset Farmasi, 2(1), 30–38. https://doi.org/10.29313/jrf.v2i1.702
Slamet, S., Anggun, B. D., & Pambudi, D. B. (2020). Uji Stabilitas Fisik Formula Sediaan Gel Ekstrak Daun Kelor (Moringa oleifera Lamk.). Jurnal Ilmiah Kesehatan, 13(2), 115–122. https://doi.org/10.48144/jiks.v13i2.260
Suradnyana, I. G. M., Widiartaa, I. M. J., & Mendra, N. N. Y. (2025). Formulasi serta Evaluasi Aktivitas Antioksidan dan Tabir Surya in Vitro Krim Ekstrak Etanol Daun Sukun (Artocarpus altilis). Usadha Jurnal Integrasi Obat Tradisional, 4(2), 76–84. https://doi.org/10.36733/usadha.v4i2.12757
Tapalina, N., Tutik, & Saputri, G. A. R. (2022). Pengaruh Metode Ekstraksi Panas Terhadap Aktivitas Antioksidan Ekstrak Kulit Bawang Merah (Allium cepa L.). Jurnal Ilmu Kedokteran Dan Kesehatan, 9(1), 492–500.
Ullah, A., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., Emwas, A., & Jaremko, M. (2020). Important flavonoids and their role as a therapeutic agent. Molecules, 25(5243), 1–39. https://doi.org/10.3390/molecules25225243
Utami, Y. P., Yulianty, R., Djabir, Y. Y., & Alam, G. (2023). Identifikasi Kandungan Senyawa dan Potensi Ekstrak Etanol 96% Daun Tekelan (Chromolaena odorata L.) sebagai Antioksidan Penangkal Radikal ABTS. Journal of Noncommunicable Disease, 8(2), 291–300. http://dx.doi.org/10.52365/jond.v3i2.785
Wahyuningsih, S., Bachri, N., Awaluddin, N., Andriani, I. (2021). Serum Wajah Fraksi Etil Asetat Daun Beluntas (Pluchea indica L.) Sebagai Antibakteri. Jurnal Katalisator, 6(2), 270–283.
Yusuf, A. L., Nurawaliah, E., & Harun, N. 2017. Uji Efektivitas Gel Ekstrak Etanol Daun Kelor (Moringa oleifera L.) sebagai Antijamur Malassezia furfur. Jurnal Ilmiah Farmasi, 5(2), 62–67. https://doi.org/10.26874/kjif.v5i2.119
Zainuddin, A., Laboko, A. I., Pade, S., & Fitriani, A. N. 2025. Optimalisasi Xanthan gum sebagai Agen Pengental Kestabilan Viskositas dan Kualitas Kecap Manis Air Kelapa. Gorontalo Agriculture Technology Journal, 8(1), 1–13.








