ANALISIS DAIDZEIN DAN GENISTEIN PADA KEDELAI (Glycine max L. Merril) VARIETAS ANJASMORO, ARGOMULYO DAN DENA 2 MENGGUNAKAN METODE KCKT
Abstract
ABSTRAK
Kedelai (Glycine max L. Merril) tidak hanya digunakan sebagai sumber protein, tetapi juga sebagai pangan fungsional yang dapat mencegah timbulnya penyakit-penyakit degeneratif, dikarenakan kandungan isoflavon. Senyawa ini dikenal memiliki kesamaan molekul dengan estrogen. Penggunaan isoflavon sebagai alternatif hormon konvesional terapi telah meningkat dalam beberapa tahun terakhir, karena aktivitas estrogenik dan efek samping yang rendah. Daidzein dan genistein merupakan isoflavon yang banyak terdapat dalam kedelai. Penelitian ini bertujuan untuk mengetahui kandungan daidzein dan genistein pada kedelai (Glycine max L. Merril) varietas Anjasmoro, Argomulyo dan Gema yang dihasilkan dari Balai Penelitian Tanaman Aneka Kacang dan Umbi (Balitkabi) Malang. Penyarian senyawa aktif digunakan metanol hasil optimasi, selanjutnya dianalisis kandungan daidzein dan genistein dengan Kromatografi Cair Kinerja Tinggi (KCKT). Sistem KCKT yang digunakan dilengkapi kolom RP-C18 Sun Fire TMC-18 (150 mm x 4,6 mm,5µm), detektor Photo Dioda Array (PDA), sistem elusi isokratik, fase gerak metanol-air yang mengandung asam asetat 0,1 % (53:47), kecepatan alir 1,0 mL/menit. Hasil penelitian menunjukkan bahwa kandungan daidzein dan genistein pada kedelai (Glycine max L. Merril) varietas Anjasmoro, Argomulyo dan Gema diperoleh kadar daidzein dan genistein masing-masing adalah 18,69 mg/100g dan 23,67 mg/100g; 29,68 mg/100 g dan 22,15 mg/100 g; 14,15 mg/100 g dan 21,22 mg/100 g.
References
Herwana, E., Setiabudy, R., Soegondo, S.,
Baziad, A., and Hidayat, A., 2015. Soy
isoflavone supplementation increases
equol-producing capability in
postmenopausal women with osteopenia.
Universa Medicina, 31: 120–130.
Hong, J.-L., Qin, X.-Y., Shu, P., Wang, Q.,
Zhou, Z.-F., Wang, G.-K., 2011.
Comparative study of isoflavones in wild
and cultivated soybeans as well as bean
products by high-performance liquid
chromatography coupled with mass
spectrometry and chemometric
techniques. European Food Research and
Technology, 233: 869–880.
Isabella da Costa César, I., Braga, F.C., Soares,
C.D.V., de Aguiar Nunan, E., Pianetti,
G.A., Condessa, F.A., 2006. Development
and validation of a RP-HPLC method for
quantification of isoflavone aglycones in
hydrolyzed soy dry extracts. Journal of
Chromatography B, 836: 74–78.
Kang, X., Jin, S., and Zhang, Q., 2009.
Antitumor and Antiangiogenic Activity of
Soy Phytoestrogen on 7, 12-Dimethylbenz
[α] anthracene-Induced Mammary
Tumors Following Ovariectomy in
Sprague–Dawley Rats. Journal of food
science, 74: H237–H242.
Kementerian Pertanian, Badan Penelitian dan
Pengembangan Pertanian, Balai
Penelitian Tanaman Aneka Kacang dan
Umbi, 2015, Deskripsi Varietas Unggul
Kedelai 1918–2014, Balai Penelitian
Tanaman Aneka Kacang dan Umbi
(Balitkabi), Malang.
Munro, I.C., Harwood, M., Hlywka, J.J.,
Stephen, A.M., Doull, J., Flamm, W.G.,
Soy isoflavones: a safety review.
Nutrition Reviews, 61: 1–33.
Parida, S., Singh, T.U., Thangamalai, R.,
Reddy, C.E.N., Panigrahi, M.,
Kandasamy, K., 2015. Daidzein
pretreatment improves survival in mouse
model of sepsis. Journal of Surgical
Research, 197: 363–373.
Pavese, J.M., Krishna, S.N., and Bergan, R.C.,
Genistein inhibits human prostate
cancer cell detachment, invasion, and
metastasis. The American journal of
clinical nutrition, 100: 431S–436S.
Pitojo Setijo, 2003. Benih Kedelai, Kanisius,
Yogyakarta.
Ravindranath, M.H., Muthugounder, S.,
Presser, N., and Viswanathan, S., 2004.
Anticancer therapeutic potential of soy
isoflavone, genistein,: Complementary
and Alternative Approaches to
Biomedicine. Springer, 121–165.
Rostagno, M.A., Villares, A., Guillamón, E.,
García-Lafuente, A., and Martínez, J.A.,
Sample preparation for the analysis
of isoflavones from soybeans and soy
foods. Journal of Chromatography A,
: 2–29.
Snyder, L.R., Kirkland, J.J., and Glajch, J.L.,
, Practical HPLC Method
Development, 2nd ed., John Wiley &
Sons, Inc., New York, 25, 653, 644, 668,
, 688, 705-706.
Suthar, A.C., Banavalikar, M.M., Biyani,
M.K., dan others, 2001. Pharmacological
activities of genistein, an isoflavone from
soy (Glycine max): Part II-Anticholesterol
activity, effects on
osteoporosis & menopausal symptoms.
Indian journal of experimental biology,
: 520–525.
Sun, J., Sun, B., Han, F., Yan, S., Hua, Y., and
Akio, K., 2011. Rapid HPLC method for
determination of 12 isoflavone
components in soybean seeds.
Agricultural Sciences in China, 10: 70–
Tipkanon, S., Chompreeda, P.,
Haruthaithanasan, V., Suwonsichon, T.,
Prinyawiwatkul, W., and Xu, Z., 2010.
Optimizing time and temperature of
enzymatic conversion of isoflavone
glucosides to aglycones in soy germ flour.
Journal of agricultural and food
chemistry, 58: 11340–11345.
Wang, H. dan Murphy, P.A., 1994. Isoflavone
content in commercial soybean foods.
Journal of agricultural and food
chemistry, 42: 1666–1673.
Yatsu, F.K., Koester, L.S., and Bassani, V.L.,
Isoflavone-aglycone fraction from
Glycine max: a promising raw material
for isoflavone-based pharmaceutical or
nutraceutical products. Revista Brasileira
de Farmacognosia, 26: 259–267.
Zhao, X.-H., Yang, Z.-Q., Bao, L.-B., Wang,
C.-Y., Gong, J.-M., Fu, C.-B., 2014.
Daidzein enhances intramuscular fat
deposition and improves meat quality in
finishing steers.
1.png){kind=small}
