UJI AKTIVITAS EKSTRAK ETANOLIK DAUN JATI (Tectona grandis L. f.) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI SECARA INVITRO
Abstract
ABSTRACT
Indonesia is a country which has a plant biodiversity, one of them is teak (Tectona grandis L.f.). Teak leaf had has flavonoids, saponins, tannin, quinones, and steroids/triterpenoids compound which have antibacterial activity. Aim of this study to determine the activities of teak leaf ethanol extract on bacteria’s growth.
The type of the research was experimental post test only control group design. There are two bacteria group, S. aureus andS. epidermidis. The treatment performed with absorbs the extract into a disc then place it over Mueller-Hinton agar which had been incubate for 24 hours with S. aureus and S. epidermidis cultures. The concentration of extract was 6,25%, 12,5%, and 25%. Clear zone was measured by calliper. Data were analyzed using One Way ANOVA and continued with Post Hoc test.
The result showed that data was distributed normally and homogeny. The highest clear zone average was obtained on S. epidermidis which has 25% extract concentrate group, and the lowest was S. aureus which has 6,25% extract concentrate group. One Way ANOVA analyze showed the value p<0,05.
It concluded, that teak leaf has an activity to inhibit bacteria’s growth, but the activity was higher on Staphylococcus epidermidis.
References
Ahmed, B., 2007,Chemistry Of Natural Products, Department of Pharmaceutical Chemistry Faculty of Science Jamia Hamdard, New Delhi.
Ajizah, A., 2004, Sensitivitas Salmonella typhimurium terhadap Ekstrak Daun Psidium guajava L., Bioscientiae, Vol.1, No.1, 31-38.
Alen, Y., Mardha, A., Isna, M., Meri, S., 2012, Uji Sitotoksik Ekstrak dan Fraksi Daun Jati (Tectona grandis linn. F.) Dengan Metoda Brine Shrimp Lethality Bioassay, Jurnal Sains dan Teknologi Farmasi, FK Universitas Andalas, Vol. 17, Hal 147-153.
Cowan, M.M., 1999, Plant Products as Antimicrobial Agent, Clinical Microbiology Reviews, 12(4): 564-582.
Departemen Kesehatan Republik Indonesia, 1985, Tanaman Obat Indonesia, Jilid II, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta.
Departemen Kesehatan Republik Indonesia, 1989, Materia Medika Indonesia, Jilid V, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta.
Departemen Kesehatan Republik Indonesia, 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta.
Dipiro, J.T., Robert L., Gary C., Barbara G., Michael P., 2005, Pharmacotherapy: A Pathophysiologic Approach, Sixth Edition, McGraw-Hill Companies Inc, USA.
Dwiprahasto I., 2005, Kebijakan untuk Meminimalkan Risiko Terjadinya Resistensi Bakteri di Unit Perawatan Intensif Rumah Sakit, Jurnal Manajemen Pelayanan Kesehatan,vol. 08 no. 04:177-180.
Hambali, E., S, Mujdalipah, A. H. Tambunan, A. W. Pattiwiri dan R. Hendroko, 2008, Teknologi Bioenergi, Agro Media, Jakarta.
Harborne, J.B., 2006, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan, Edisi ke-2, ITB, Bandung.
Hartati, R., S. A. Gana., dan K. Ruslan., 2005, Telaah flavonoid dan Asam Fenolat Daun Jati (Tectona grandis L. f., verbenaceae), Skripsi, Institut Teknologi Bandung, Bandung.
Katzung, B. G., 2007, Basic & Clinical Pharmacology, Tenth Edition, Lange Medical Publications, United States.
Kravitz GR, Dries DJ, Peterson ML., 2005. Purpura Fluminans due to Staphylococcus aureus, Clinical Infection Disease. [Medline]
Lestari, A., Jamhari, M., Nengah, K., 2013, Daya Hambat Ekstrak Daun Tembelek (Lantana camara L.) terhadap Pertumbuhan Escherichia Coli, e-Jipbiol, Universitas Tadulako, Vol 1, Hal 42 – 49.
Marsusi, Ahmad D.S., Shanti L., 2001, Studi Kemotaksonomi pada Genus Zingiber, Jurnal Biodiversitas, Vol. 2, No. 1, Hal. 92-97.
Puji, N.A., 2009, Sifat Organoleptik Tempe Kedelai yang Dibungkus Plastik, Daun Pisang, dan Daun Jati, Skripsi, UMS.
Simmons BP, Gelfand MS, Grogan J, Craft B., 1995, Cefotaxime twice daily versus ceftriaxone once daily, A randomized controlled study in patients with serious infections, Diagnostic Microbiology and Infectious Disease, 22 (1–2), 155–7.
Saising, Jongkon, Linda D., Anne-Kathrin Z., Supayang PV., Mulugenta N., Friedrich G., 2012, Activity of Gallidermin on Staphylococcus aureus and Staphylococcus epidermidis Biofilms.Antimicrob, Agents Chemoter, Vol. 56, No. 11, Hal. 5804-5810.
Suliantari, 2009, Aktivitas Antibakteri dan Mekanisme Penghambatan Ekstrak Sirih Hijau (Piper btle Linn) Terhadap Bakteri Patogen Pangan, Disertasi, Jurusan Ilmu Pangan, Sekolah Pasca Sarjana IPB, Bogor.
Syukur, C., dan Harnani, 2002, Budi Daya Tanaman Obat Komersial, Kanisius, Yogyakarta, hal 91.
WHO, 2015, The International Pharmacopoeia, 5th Edition, World Health Organization Cataloguing-in-Publication Data, Geneva, Printed in Singapore.
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